Figure 1. A – Kinetics of siRNA-mediated CT-X knockdown: SK-MEL-37 cells were transfected separately with10 nM of scrambled, GAGE#9 and #15 siRNAs and cells were harvested for real-time PCR 3, 6, 12, 18, 24 and 48 hours after transfection.

Relative quantification of gene expression (relative amount of target RNA) was determined using the equation 2^−ΔΔC_T using the sample transfected with scrambled siRNA as calibrator.

B: Efficiency of siRNA-mediated CT-Xp knockdown: Western blot analysis was used to examine the effect of the specific siRNAs on CT-Xp expression at the protein level. Reduction of protein levels to almost complete depletion was present 72 hours after transfection with all tested siRNAs.