Table 1. Characteristics of the 27mer siRNA duplexes designed for this study.
| Duplex name | Sense sequence (5'-3') | Antisense sequence (5'-3') | RefSeq | Sense position | Antisense position | Specificity2 |
|---|---|---|---|---|---|---|
| GAGE#9 | GUUCAGUGAUGAAGUGGAACCAGCA | UGCUGGUUCCACUUCAUCACUGAACUG | NM_001468 | 209 | 233 | GAGE1,2,8,10,12 |
| GAGE#15 | GAACCAGCAACUCAACGUCAGGATC | GAUCCUGACGUUGAGUUGCUGGUUCCC | NM_001468 | 249 | 273 | GAGE1,2,8,10,12 |
| SSX#12 | CAAGGUCACCCUCCCACCUUUCATG | CAUGAAAGGUGGGAGGGUGACCUUGAA | NM_005636 | 247 | 271 | SSX4, SSX4B |
| SSX#19 | CUUGUGUAUCCAUGCACCUACCUCA | UGAGGUAGGUGCAUGGAUACACAAGCC | NM_005636 | 892 | 916 | SSX4, SSX4B, SSX6 |
| XAGE1 #2 | GACAGAAGAAGAUCAGGAUACAGCT | AGCUGUAUCCUGAUCUUCUUCUGUCUG | NM_133430 | 197 | 221 | XAGE1 |
| XAGE1 #9 | AAGCUGAAACAACGCAAGCUGGUTT | AAACCAGCUUGCGUUGUUUCAGCUUGU | NM_133430 | 406 | 430 | XAGE1 |
1
The sense strand has two terminal 3' nucleotides as DNA, and the remainder bases as RNA for preferential uptake of the antisense strand into RISC (RNA induced silencing) complex.
2
Only genes presenting 100% identity with the siRNA, as assessed with the BLAST tool at NCBI are listed.