Table 1. Characteristics of the 27mer siRNA duplexes designed for this study.
Duplex name | Sense sequence (5'-3') | Antisense sequence (5'-3') | RefSeq | Sense position | Antisense position | Specificity2 |
---|---|---|---|---|---|---|
GAGE#9 | GUUCAGUGAUGAAGUGGAACCAGCA | UGCUGGUUCCACUUCAUCACUGAACUG | NM_001468 | 209 | 233 | GAGE1,2,8,10,12 |
GAGE#15 | GAACCAGCAACUCAACGUCAGGATC | GAUCCUGACGUUGAGUUGCUGGUUCCC | NM_001468 | 249 | 273 | GAGE1,2,8,10,12 |
SSX#12 | CAAGGUCACCCUCCCACCUUUCATG | CAUGAAAGGUGGGAGGGUGACCUUGAA | NM_005636 | 247 | 271 | SSX4, SSX4B |
SSX#19 | CUUGUGUAUCCAUGCACCUACCUCA | UGAGGUAGGUGCAUGGAUACACAAGCC | NM_005636 | 892 | 916 | SSX4, SSX4B, SSX6 |
XAGE1 #2 | GACAGAAGAAGAUCAGGAUACAGCT | AGCUGUAUCCUGAUCUUCUUCUGUCUG | NM_133430 | 197 | 221 | XAGE1 |
XAGE1 #9 | AAGCUGAAACAACGCAAGCUGGUTT | AAACCAGCUUGCGUUGUUUCAGCUUGU | NM_133430 | 406 | 430 | XAGE1 |
The sense strand has two terminal 3' nucleotides as DNA, and the remainder bases as RNA for preferential uptake of the antisense strand into RISC (RNA induced silencing) complex.
Only genes presenting 100% identity with the siRNA, as assessed with the BLAST tool at NCBI are listed.