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. 2013 Jul 23;8(7):e69413. doi: 10.1371/journal.pone.0069413

Figure 5. Polymers do not affect DC activation by encapsulated viruses.

Figure 5

(A) Polymers do not affect TNFα production post VSV stimulation. DCs were generated form bone marrow cells of WT animals cultured in the presence of IL4 and GMCSF for 7 days. Cells were then stimulated with live VSV at a multiplicity of infection (MOI) of 1 in the presence of 20 µg/mL PAMAM-G3. Culture supernatants were analyzed for secretion of IL6 by ELISA, 24 h later. (B) Polymers do not affect TNFα production post vaccinia stimulation. DCs were generated form bone marrow cells of WT animals cultured in the presence of IL4 and GMCSF for 7 days. Cells were then stimulated with live vaccinia at a multiplicity of infection (MOI) of 1 in the presence of 20 µg/mL PAMAM-G3. Culture supernatants were analyzed for secretion of IL6 by ELISA, 24 h later. (C) Polymers do not affect IFNα production post VSV stimulation. pDCs were generated from bone marrow cells of WT animals in the presence of Flt-3L for 10 days. Cells were then stimulated with live VSV at an MOI of 1 in the presence of 20 µg/mL PAMAM-G3. Supernatants were analyzed for the presence of IFNα by ELISA 24 h later. Data are presented as mean+/− SD and are representative of at least 3 independent experiments. n = 9 mice per group. (D) Polymers do not affect IL6 production post VSV stimulation as compared to TLR inhibitors. DCs were generated form bone marrow cells of WT animals cultured in the presence of IL4 and GMCSF for 7 days. Cells were then stimulated with live VSV at an MOI of 1 in the presence of 20 µg/mL PAMAM-G3, CLI-095 and IRS954. Supernatants were analyzed for the presence of IL6 by ELISA 24 h later. Data are presented as mean+/− SD and are representative of at least 3 independent experiments. n = 9 mice per group. *p<0.05.