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A ROS1 break-apart fluorescent in situ hybridization (FISH) assay. FISH reveals separation of the 5′ ROS1 probe (green) from the 3′ ROS1 probe (red), indicative of a ROS1 rearrangement in a patient with non-small cell lung cancer. Size bar = 10 μm. Reprinted from [25] with permission [Bergethon, K et al: J Clin Oncol 2012;30:863—870 © 2013 American Society of Clinical Oncology. All rights reserved].
