Table 2. Kinetic parameters for YfdE and UctC.a .
Substrate | Parameter | YfdEb | H6YfdE | YfdEH6 | UctC |
acetyl-CoAc |
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11 | 12 | 6.9 | 2.0 |
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17 | 18 | 13 | 81 | |
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|
oxalated |
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15 | 22 | 10 | 1.8 |
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22 | 39 | 22 | 1.0 | |
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Saturation curves are shown in the Supporting Information (Figure S3). values were computed assuming one active site per subunit and individual subunit masses (Table S1).
No correction was applied to account for the lower purity of YfdE ().
Determined at 50 mM oxalate. This concentration is subsaturating for YfdE forms; the and
values given are likely to be lower than the true values.
Determined at 0.75 mM acetyl-CoA.