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. 2013 Jul 23;8(7):e67901. doi: 10.1371/journal.pone.0067901

Table 2. Kinetic parameters for YfdE and UctC.a .

Substrate Parameter YfdEb H6YfdE YfdEH6 UctC
acetyl-CoAc Inline graphic (Inline graphic) 11 12 6.9 2.0
Inline graphic (Inline graphicM) 17 18 13 81
Inline graphic (Inline graphic) Inline graphic Inline graphic Inline graphic Inline graphic
oxalated Inline graphic (Inline graphic) 15 22 10 1.8
Inline graphic (mM) 22 39 22 1.0
Inline graphic (Inline graphic) Inline graphic Inline graphic Inline graphic Inline graphic
a

Saturation curves are shown in the Supporting Information (Figure S3). Inline graphic values were computed assuming one active site per subunit and individual subunit masses (Table S1).

b

No correction was applied to account for the lower purity of YfdE (Inline graphic).

c

Determined at 50 mM oxalate. This concentration is subsaturating for YfdE forms; the Inline graphic and Inline graphic values given are likely to be lower than the true values.

d

Determined at 0.75 mM acetyl-CoA.