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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Prostate. 2013 May 9;73(12):1352–1363. doi: 10.1002/pros.22683

Fig. 7.

Fig. 7

Expression of unphosphorylatable mutants of CLIP-170 inhibits microtubule dynamics. U2OS cells stably expressing GFP-CLIP-170 constructs (WT, S195A, S195A/S1318A or T287A) were grown on coverslips and analyzed for GFP signal by variable-angle epifluorescence microscopy (VAEM). Images were taken every 10 seconds. Ten cells from each group were recorded and 5 assembled microtubules from each cell were measured for time of duration (lifetime) of assembled CLIP-170 protein (A), the maximum length of microtubules (B), and the rate of CLIP-170 protein assembly (growth) (C). In comparison with cells expressing GFP-CLIP170-WT, statistical significance of cells expressing CLIP-170-S195A, -S195/S1318A and -T287A was calculated using SPSS Statistics 17.0 (t test). P < 0.01 was considered statistically significant. For all panels, statistical values are **, P < 0.005; ***, P < 0.001.