Figure 6. TLR-4 and -9 ligands improve the IFNγ memory response of effector T cells.

CFSE-positive total (D2-8) lymph node effector T cells were sorted and re-transferred into Rag2^−/− hosts. On day 1, 5, and 8 after cell transfer, TLR ligands were administered i.p. The spleen cells were harvested 5 weeks after the last TLR injection and the percentage and the cell number as well as IFNγ production by the surviving CD4⁺KJ1-26⁺ cells were measured by flow cytometry and ELISA, respectively. The cell number was relative to PBS (set at 1 ± 0.1 × 10⁴). Each bar represents the mean of nine independent experiments. *p< 0.05, **p< 0.01.