Table 2. Results of quantitative real-time PCR analysis of the 8 confirmed CNVRs.
| CNVR _ID | Chr. | Strarta | Enda | Freq. | Type | Primer | Predicted positive samples | Predicted negative samples | Validated | ||||
| Sample | Sample | Positive | Sample | Sample | negative | ||||||||
| detected | confirmed | predictive rate | detected | confirmed | predictive rate | ||||||||
| 7 | 1 | 296533542 | 296809518 | 0.1429 | gain | E1-2 | 2 | 2 | 1 | 11 | 9 | 0.8182 | Y |
| E2-1 | 2 | 2 | 1 | 11 | 11 | 1 | Y | ||||||
| 50 | 7 | 50327355 | 50750507 | 0.0714 | loss | D1-1 | 1 | 1 | 1 | 12 | 0 | 0 | Y |
| D2-1 | 1 | 1 | 1 | 12 | 0 | 0 | Y | ||||||
| 53 | 7 | 122956303 | 123104663 | 0.1429 | gain | H1-1 | 2 | 1 | 0.5 | 11 | 2 | 0.1818 | Y |
| H2-2 | 2 | 2 | 1 | 11 | 2 | 0.1818 | Y | ||||||
| 11 | 11 | 62514730 | 62841029 | 0.1429 | loss | F1-2 | 2 | 2 | 1 | 11 | 0 | 0 | Y |
| F1-3 | 2 | 2 | 1 | 11 | 0 | 0 | Y | ||||||
| 12 | 11 | 70508069 | 71089190 | 0 | gain | J1-3 | – | – | – | 11 | 7 | N | |
| J2-1 | 2 | 2 | 1 | 11 | 4 | 0.3636 | y | ||||||
| 13 | 11 | 84740020 | 84849706 | 0.0714 | loss | M1-2 | 1 | 1 | 1 | 12 | 4 | 0.3333 | Y |
| M2-1 | 1 | 1 | 1 | 12 | 4 | 0.3333 | Y | ||||||
| 20 | 14 | 3815879 | 3862162 | 0.1429 | loss | B1-1 | 2 | 0 | – | – | 11 | 0 | N |
| B2-1 | 2 | 1 | 0.5 | 11 | 5 | 0.4545 | Y | ||||||
| 27 | 15 | 13218467 | 13325812 | 0.2857 | loss | L1-1 | – | – | – | 9 | – | N | |
| L1-3 | 4 | 4 | 1 | 9 | 6 | 0.6667 | Y | ||||||
| Average | 0.9231 | 0.3333 | |||||||||||
a
The Sus scrofa assembly (10.2) (http://www.ensembl.org/Sus_scrofa/Info/Index) was used to indicate the position of the CNVRs.