Table 1.

Cyclopropanation activities for intact E. coli cells expressing engineered enzymes.

Entry	Catalyst	[EDA] (mM)	Cell Density (gcdw/L)	[P450] (3M)	Yield (%)	TTN	cis:trans	eecis (%)*	eetrans (%)†
1	P450BM3-CIS	8.5	7.7	3.7	42	950	22:78	60	22
2	P411BM3-CIS	8.5	7.7	1.3	55	3,700	76:24	96	25
3	P411BM3-heme-CIS	8.5	7.7	3.6	67	1,600	71:29	95	17
4	P450BM3	8.5	13.4	4.8	0.9	15	25:75	24	21
5	P411BM3	8.5	8.1	1.5	50	2,900	13:87	12	8
6	P411BM3-CIS + CO	8.5	8.4	1.8	0.6	30	20:80	35	20
7	P411BM3-CIS‡	170	8.4	1.8	72	67,800	90:10	99	43
8	P411BM3-CIS‡	200	20	3.2	78§	48,800	88:12	99	35

P411_BM3 = P450_BM3-C400S

TTN = total turnover number. Major products:

^*cis = (1R,2S);

^†trans = (1S,2S)

^‡Neat reagents were used without addition of MeOH; reactions were left for 24 h.

^§Isolated yield (1.63 g cyclopropanes). The data represent the averages of triplicate experiments. Standard errors are within 20% of the reported average.