Sera were collected from the (A–D) NP-KLH/Alum-immunized and (E–H) NP-KLH/α-GC-immunized chimeras on the ds indicated. Samples were analyzed by ELISA and endpoint anti-NP IgG1 titers determined. Data shows mean endpoint titer ±SD. Checkered bars indicate sera collected before the booster vaccine, whereas solid bars represent sera collected after the booster. Titers in pre-bleed sera were below 1/200 (not depicted). Numbers of mice per group were: (A, D, E, H) n = 7–11, (B, C, F, G) n = 4 – 6. Statistically significant differences between experimental groups were determined by one-way ANOVA and Dunn’s post-test.