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. 2013 Jul 23;8(7):e67597. doi: 10.1371/journal.pone.0067597

Figure 3. Confocal laser-scanning microscopy.

Figure 3

Bright field (Aa, Ac and Ae) and confocal fluorescence (Ab, Ad and Af) micrographs of C. albicans cells treated with FITC-conjugated Anal 3 (Aa and Ab), Anal 3-Pro (Ac and Ad) or magainin II (Ae and Af). Bright field (Ba, Bc, Be and Bg) and confocal fluorescence (Bb, Bd, Bf and Bh) micrographs of T. begellii cells treated with FITC-conjugated Anal 3 (Ba and Bb), Anal 3-Pro (Bc and Bd), buforin II (Be and Bf) or TAT (Bg and Bh). Cells were incubated for 15 min at 28°C with the respective peptides. Note that whereas FITC-Anal 3-Pro, buforin II and TAT are distributed relatively uniformly in the cytoplasm, FITC-Anal 3 and FITC-Magainin II are localized to the periphery, presumably associated with the cell membrane.