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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nat Chem Biol. 2013 Jun 2;9(8):514–520. doi: 10.1038/nchembio.1270

Fig. 2. Expansion of primary human hepatocytes.

Fig. 2

(a) Primary screening data for FPH1 and FPH2. Data presented as mean ± s.d. (b) Ki67 (red) and albumin (green) immunofluorescent staining after 6 days of culture. Bar graphs show CellProfiler quantification of displayed images. (c) FACS (right) and Cellometer (left) Automated Cell Counter analysis. Fibroblasts were labeled with CM-DiI prior to initiation of culture in order to allow identification of hepatocytes via negative selection. FACS cell counting was further enabled by fluorescent counting beads. Control cultures were treated with empty vehicle (DMSO). Data presented as mean ± SEM.