Fig. 3. Functional enhancement of human primary hepatocytes and iHeps.
(a) Primary screening data of FH1. (b) Primary screening data of FPH1. Control cultures (Ctrl) were treated with empty vehicle (DMSO). All data presented as mean ± s.d. (c) Morphology and colony size of FPH1- and FH1-treated iHeps in 6-well plates 9 days post-initiation-of-treatment. Untreated iHeps are shown for comparison (scale bar = 100 µm). Yellow arrows point to select bile canaliculi. (d) Albumin, CYP3A and AFP staining of iHeps after 9 days of culture (scale bar = 100um). Bar graphs represent quantifications of displayed images.