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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Eur J Med Chem. 2013 Apr 2;65:83–93. doi: 10.1016/j.ejmech.2013.03.035

Figure 3. UNC1062 inhibits Mer activation in adherent tumor cell lines.

Figure 3

A) BT-12 pediatric rhabdoid tumor cells were treated with UNC1062 or vehicle only for 1 h prior to the addition of pervanadate for 15 min. Cell lysates were prepared, Mer protein was immunoprecipitated, and phospho-tyrosine and Mer protein were detected by western blot. B) The indicated NSCLC cell lines were treated with UNC1062 or an equivalent volume of vehicle only for 72 h prior to treatment with pervanadate for 1.0 (Colo699) or 5.0 (A549) min. Whole cell lysates were prepared, Mer protein was immunoprecipitated, and phosphorylated and total Mer proteins were detected by western blot. The results shown are representative of 3 independent experiments.