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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Microbiol Methods. 2013 May 12;94(2):77–82. doi: 10.1016/j.mimet.2013.05.005

Fig. 4. Cloning-independent construction of pZX7 in other streptococci.

Fig. 4

(A) POE-PCR was used to generate concatemers of the pDL278 streptococcal replicon and a gusA reporter fusion. The concatemers were transformed into S. sanguinis SK36 and S. gordonii DL1 and then assayed for the number of pZX7 transformants per μg of concatemer DNA. The averages of three independent experiments are shown. (B) The concatemer PCR reaction was transformed into S. sanguinis, diluted 100-fold, and plated on agar plates containing spectinomycin and X-Glu. (C) The concatemer PCR reaction was transformed into S. gordonii, diluted 10-fold, and plated on agar plates containing spectinomycin and X-Glu.