Table 2.
Primer | Sequence (5′ → 3′) | Purpose |
---|---|---|
pRF-pDL TCTTAGAC | CACGAACGAAAATCGATCTTCTCACTAATGGTTACC | pZX7 construction and confirmation |
gusAR-pDL | ACGAGGCTAGTTTACCGTATCTTCATTGTTTGCCTCCCTGCTGC | pZX7 construction and confirmation |
pDLF-PR | GTCTAAGAGGTAACCATTAGTGAGAAGATCGATTTTCGTTCGTG | pZX7 construction |
pDLR-gusA | GCAGCAGGGAGGCAAACAATGAAGATACGGTAAACTAGCCTCGT | pZX7 construction |