Fig. 4.

TLR8 stimulation of PBMC in presence of cetuximab-coated HNC cells causes inflammatory cytokine secretion. PBMC were incubated with TLR8 selective agonist VTX-2337 (250 nM) or media for 18 h, then co-cultured at E:T = 50:1 for 4 h with cetuximab (10 μg/mL)-coated UM-22B HNC cells. Supernatants were collected from untreated control and TLR8-stimulated conditions. TNFα, IFNγ, and IL-12p40 secretion was significantly enhanced (p < 0.0001, p < 0.0001, and p < 0.005 respectively). Of note, IL-12p40 enhancement was still significant when analyzed without the single high outlier (p < 0.01). **p < 0.005, ***p < 0.0001