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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Cancer Res. 2013 Apr 24;11(7):793–807. doi: 10.1158/1541-7786.MCR-12-0600

Figure 3. S1P and C1P increase the adhesivness of RMS cells and induce F-actin formation and translocation of paxillin to focal adhesion sites.

Figure 3

Panel A - Adhesion of RMS cells to fibronectin. The cells were not stimulated (control) or stimulated with S1P (0.1 or 1 μM) or C1P (0.5 or 1 μM) for 1 h. The number of adherent cells was measured by microscopic analysis. Data from three separate experiments are pooled together and means ± SD are shown. *p < 0.05 and **p < 0.01 compared with the control. Panel B - Adhesion of RH30 and RD cells to mouse stromal cells. RMS cells stained with calcein were stimulated with S1P (1 μM) or C1P (0.5 μM) for 1 h. After a 15-minute incubation, non-adherent cells were removed and adherent cells counted under a fluorescent microscope. Panel C - Actin cytoskeleton organization and paxillin localization in RH30 and RD cells in medium alone or after 4-h stimulation with 1 μM S1P or 0.5 μM C1P. Representative images are shown.