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. 2013 Jul 23;8(7):e68916. doi: 10.1371/journal.pone.0068916

Figure 1. LCI measures mass of T and target cells.

Figure 1

(A) Workflow for T cell mass measurement experiments. Donor peripheral blood mononuclear cells (PBMCs) are transduced with the MART1 specific, F5 TCR and enriched for CD8+ T cells. A subset of these T cells are analyzed by flow cytometry to confirm a transduction efficiency of greater than 50%. The remaining cells are imaged on the LCI with MART1 expressing, HLA-matched (or mismatched control) M202 target cells. (B) Sample LCI data showing the phase shift and mass distributions in an activated, F5-transduced CD8+ T cell, several unresponsive T cells, and a dying target cell.