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. 2013 Jul 23;8(7):e68916. doi: 10.1371/journal.pone.0068916

Figure 2. Transduction of CD8+ enriched PBMCs.

Figure 2

(A) Flow cytometry data of transduced T cells showing typical transduction efficiency of donor PBMCs. (B) Flow cytometry of CD8+ enriched population showing CD8+ T-cell enrichment efficiency. (C) IFNg release assay validating F5-transduced, CD8+ enriched T cell activation following co-culture with HLA-matched MART1 expressing M202 cells. Negative control M207 cells express MART1, but are HLA-mismatched. (D) Mass vs. time of the healthy M202 cell shown in (E), demonstrating the viability of target cells on the interferometer stage.