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. 2013 Jul 23;8(7):e68875. doi: 10.1371/journal.pone.0068875

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© 2013 Lofton et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Competitions between the reconstituted waaY frameshift mutant DA22427 (cfp tagged) and its congenic, susceptible counterpart DA22431 (yfp tagged). These experiments were performed at different concentrations of the three different peptides in this study, (A) WGHs (orange), (B) LL-37 (purple) and (C) CNY100HL (green). The curves represent the ratio of resistant (waaY mutant) to susceptible strain at different generations of growth. The data is the result of at least 2 separate competitions performed in duplicate for each peptide. (D) The selection coefficient ± SEM was deduced from the slopes in (A)-(C) and is plotted as a function of peptide concentration.