Table 3. Fitness of original and reconstituted mutants relative to wild type controls as determined by growth rate assays and competition experiments.
| Strain | Fitness change relative to wild type | |||
| Growth rate assay | Competition assay | |||
| Refined LB | MH | NaPB | NaPB | |
| Wild type controlsa | 1 | 1 | 1 | 1 |
| DA16875 (WGH resistant isolate 1) | 0.89 | 0.87 | 0.88 | ND |
| DA16874 (LL-37 resistant isolate 1) | 0.88 | 0.94 | 0.91 | ND |
| DA17847 (LL-37 resistant isolate 2) | 0.97 | 0.96 | 0.90 | ND |
| DA17610 (CNY100HL resistant isolate 1) | 0.83 | 0.75 | 0.73 | ND |
| DA22427 (waaY [FS] reconstituted) | ND | 0.96 | 1.05 | 0.99 |
| DA23175 (pmrB [R13H] reconstituted) | ND | 0.99 | 1.04 | 1.01 |
| DA23307 (phoP [D23N] reconstituted) | ND | 0.93 | 0.84 | 0.84 |
| DA23899 (waaY, pmrB, phoP reconstituted) | ND | 0.83 | 0.91 | 0.84 |
a
DA6079 was used as wild type control for the original mutants and the respective congenic strains were used as controls for reconstituted mutants.
ND, not determined.
Maximum standard error of the mean (SEM): Refined LB (SEM ±0.02), MH (SEM ±0.03) and NaPB (SEM ±0.05).