Figure 2. AKAP3 degradation is dependent on capacitation conditions and proteasome activity.

Bovine spermatozoa were incubated for 4h or 7h in the mTALP capacitation medium (A and B) or NKM medium (non-capacitating medium) (A), in the presence or absence of MG-132 (10 µM) (B). Proteins were extracted and analyzed by western blot using anti-AKAP3 and anti-β-actin antibodies. The results represent three independent experiments. Note: The bands from the sides of the cut are from the same blot. We see the cut because few irrelevant bands were taken out of the picture.