Figure 6. AKAP3 degradation is independent of PKA activation, but depends on PKA anchoring.

Bovine spermatozoa were incubated in capacitation medium with or without bicarbonate, H89 (50 µM) or 8Br-cAMP (1 mM) (A,B). H89 was added at the beginning of capacitation for 10 min, followed by addition of 8Br-cAMP. Samples were removed at the beginning of the assay, and after 4h of capacitation. Proteins were extracted and analyzed by western blot using anti-AKAP3 antibody and anti-β-actin antibodies (loading control). The results represent three independent experiments.