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. 2013 Jul 23;8(7):e68873. doi: 10.1371/journal.pone.0068873

Figure 7. Disruption of PKA anchoring, but not AKAP3 degradation, affects PKARII state.

Figure 7

Bovine spermatozoa were incubated in capacitation medium. Ht31 (5 µM), Ht31-P (5 µM) (control peptide) (A,C), NH4Cl (10 mM) (B,C), H89 (50µM) and 8Br-cAMP (1 mM) (B) were added at the beginning of capacitation. Samples were taken out at the beginning and after 4h of capacitation. Proteins were extracted and analyzed by western blot using anti-AKAP3 (A,B,C), anti-AKAP95 (B), anti PKARII (C), and anti-β-actin or anti-α-tubulin antibodies (loading control). The results represent three independent experiments. Note: The bands from the sides of the cut are from the same blot. We see the cut because few irrelevant bands were taken out of the picture.