Figure 2. Topology representation of the predicted membrane proteins in the “pan-surfome” of the 16 S. pneumoniae clinical isolates that have been identified in at least 50% of the analyzed strains.

The TMHMM algorithm was used to predict transmembrane domains (TMD) and signal peptides after prediction of subcellular localization by LocateP. In red are shown the peptides experimentally identified by LC/MS/MS. A) Proteins with only one predicted TMD. B) Proteins with more than one TMD.