Table 3. Surface proteins identified in ≥50% clinical isolates by “shaving” Streptococcus pneumoniae cells followed by LC/MS/MS analysis.
Clinical Isolates | ||||||||||||||||||
Locus | Description | 5074 | 5104 | 5231 | 5278 | 5284 | 5330 | 5333 | 5334 | 5335 | 5337 | 5341 | 5342 | 5343 | 5344 | 5345 | 5431 | # Isolates |
Lipoproteins a | ||||||||||||||||||
spr0884 | foldase protein PrsA | × | × | × | × | × | × | × | × | 8/16 | ||||||||
spr1060 | pneumococcal histidine triad protein D precursor | × | × | × | × | × | × | × | × | × | 9/16 | |||||||
spr1382 | ABC transporter substrate-binding protein - oligopeptide transport (AliB) | × | × | × | × | × | × | × | × | × | 9/16 | |||||||
Cell Wall | ||||||||||||||||||
spr0057 | beta-N-acetylhexosaminidase | × | × | × | × | × | × | × | × | × | × | × | × | × | × | 14/16 | ||
spr0075 | cell wall surface anchor family protein | × | × | × | × | × | × | × | × | 8/16 | ||||||||
spr0247 | alkaline amylopullulanase | × | × | × | × | × | × | × | × | × | × | × | 11/16 | |||||
spr0286 | hyaluronate lyase precursor (hyaluronidase/hyase) | × | × | × | × | × | × | × | × | × | × | × | × | 12/16 | ||||
spr0328 | cell wall surface anchor family protein | × | × | × | × | × | × | × | × | × | × | × | × | × | × | × | 15/16 | |
spr0440 | endo-beta-N-acetylglucosaminidase, putative | × | × | × | × | × | × | × | × | × | × | × | × | 12/16 | ||||
spr0561 | cell wall-associated serine proteinase precursor PrtA | × | × | × | × | × | × | × | × | × | × | × | × | × | 13/16 | |||
spr0565 | beta-galactosidase precursor | × | × | × | × | × | × | × | × | × | × | × | 11/16 | |||||
spr1403 | hypothetical protein spr1403 | × | × | × | × | × | × | × | × | × | × | × | × | × | 13/16 | |||
spr1536 | sialidase A precursor (neuraminidase A) | × | × | × | × | × | × | × | × | × | × | × | × | × | 13/16 | |||
Membrane proteins (1TMD) | ||||||||||||||||||
spr0121 | surface protein pspA precursor | × | × | × | × | × | × | × | × | × | × | × | × | × | × | 14/16 | ||
spr0329 | penicillin-binding protein 1A | × | × | × | × | × | × | × | × | 8/16 | ||||||||
spr0334 | hypothetical protein spr0334 | × | × | × | × | × | × | × | × | 8/16 | ||||||||
spr0907 | pneumococcal histidine triad protein D precursor | × | × | × | × | × | × | × | × | × | × | × | × | × | × | 14/16 | ||
spr1370 | hypothetical protein spr1370 | × | × | × | × | × | × | × | × | × | × | 10/16 | ||||||
spr1875 | hypothetical protein spr1875 | × | × | × | × | × | × | × | × | 8/16 | ||||||||
spr2045 | serine protease | × | × | × | × | × | × | × | × | × | × | × | 11/16 | |||||
Membrane proteins (>1TMD) | ||||||||||||||||||
SP_0071 | immunoglobulin A1 protease | × | × | × | × | × | × | × | × | × | 9/16 | |||||||
spr0012 | cell division protein FtsH | × | × | × | × | × | × | × | × | × | × | × | × | 12/16 | ||||
spr0086 | hypothetical protein spr0086 | × | × | × | × | × | × | × | × | × | × | × | × | 12/16 | ||||
spr0581 | Zinc metalloprotease | × | × | × | × | × | × | × | × | × | × | × | × | × | × | × | × | 16/16 |
spr1042 | immunoglobulin A1 protease | × | × | × | × | × | × | × | × | × | × | × | × | × | × | × | × | 16/16 |
SPN23F_10590 | Zinc metalloprotease ZmpD | × | × | × | × | × | × | × | × | 8/16 | ||||||||
SP670_2141 | TMP repeat family | × | × | × | × | × | × | × | × | 8/16 | ||||||||
Secretory | ||||||||||||||||||
spr0908 | pneumococcal histidine triad protein E precursor | × | × | × | × | × | × | × | × | × | × | 10/16 | ||||||
spr0931 | hypothetical protein spr0931 | × | × | × | × | × | × | × | × | × | × | × | 11/16 | |||||
spr1903 | UTP-glucose-1-phosphate uridylyltransferase | × | × | × | × | × | × | × | × | × | × | 10/16 | ||||||
spr1995 | choline binding protein A | × | × | × | × | × | × | × | × | × | × | × | × | × | × | × | × | 16/16 |
spr2021 | general stress protein GSP-781 | × | × | × | × | × | × | × | × | × | × | × | × | 12/16 |
Protein categories were established according to LocateP subcellular predictions: lipoproteins were those predicted as lipid-anchored proteins; cell wall proteins, as those having an LPXTG motif; secretory proteins, as those possessing an SP1-type signal peptide; membrane proteins with one transmembrane domain (TMD), as those possessing either a C- or an N-terminally anchored transmembrane region; membrane proteins with more than one transmembrane domain, those predicted as multi-transmembrane proteins; “surface proteins” means the sum of the five previous categories; and cytoplasmic proteins, those without any exporting or sorting signal, and predicted as intracellular proteins.