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. 2013 Jul 23;8(7):e70218. doi: 10.1371/journal.pone.0070218

Figure 5. D466E Orco is more sensitive to odorants when expressed together with a tuning Or.

Figure 5

(A,B) Flp-In 293 T-REX cells stably transfected with WT, D476E and D476N DmelOrco were transfected with pCI-DmelOr22a. Orco was induced with tetracycline (0.3 µg/ml) and calcium fluorescence determined before and after the addition of methyl hexanaoate. (A) Time-course of calcium fluorescence following addition of 10 µM methyl hexanaote. (B) Concentration-response curves of WT D466E and D466N DmelOrco: DmelOR22a complexes to methyl hexanaote. LogEC50 values for WT (−6.38±0.018) and D466E (−6.81±0.03) differed significantly (**p<0.001), as did WT and D466N (−6.09±0.01). (C) Concentration-response curves of WT, D466E, and D466N DmelOrco cells transfected with AgOR65 to eugenol. LogEC50 values for WT (−6.54±0.05) and D466E (−6.75±0.03) differed significantly (**p<0.001), while D466N (−6.34±0.04) did not.