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. 2013 Jul 23;8(7):e70440. doi: 10.1371/journal.pone.0070440

Figure 1. 2-D PAGE reference map of surface-exposed proteins in R. heilongjiangensis.

Figure 1

R. heilongjiangensis biotinylated proteins isolated by biotin-streptavidin affinity purification were separated using a pH 3–10 NL IPG strip (Bio-Rad, Richmond, CA) in the first dimension followed by 12% SDS-PAGE. Numbers with arrowheads refer to the protein spots in the silver-stained gel. Protein spots excised from the gel were digested and subjected to ESI-MS/MS analysis. The relative molecular masses of the marker proteins are indicated in kDa on the left side of the figure and the protein spots identified by ESI-MS/MS analysis are listed in Table S2.