Table 3. Serological specificity of the major seroreactive surface-exposed proteins of R. heilongjiangensis analyzed by microarray assay using serum samples from mice infected with different rickettsial agents.
| Average and standard deviation (SD) of the FI value of each protein probed with different serum samples (positive No./total No.) | ||||||||
| Proteins | R. hei | R. sib | R. ric | R. pro | R. typ | O. tsu | C. bur | Normal |
| GroEL | 727±434.5 (10/10) | 281±569.9 (6/10) | 29.7±35.4 (1/10) | 10.2±18.5 (0/10) | 25.6±18.3 (0/10) | 36.6±28.8 (2/10) | 15.5±9.9 (0/10) | 19.1±15 (0/10) |
| OmpA-2 | 3629.7±2294 (10/10) | 464±218 (10/10) | 78±33.5 (0/10) | 80.6±47.9 (2/10) | 953.2±258.2 (10/10) | 38.0±22.0 (0/10) | 58.9±33.5 (0/10) | 61.9±25.6 (0/10) |
| OmpB-3 | 1038.1±629.8 (9/10) | 253±189 (10/10) | 69.3±36.2 (2/10) | 44±40.2 (0/10) | 86.1±21 (1/10) | 38.9±28.9 (0/10) | 33±14.8 (0/10) | 45.2±20.8 (0/10) |
| PrsA | 745.6±1296.1 (8/10) | 112±48 (2/10) | 45.3±34.4 (0/10) | 62.1±34.0 (0/10) | 66.4±18.5 (0/10) | 43.1±39.2 (0/10) | 30.8±27.1 (0/10) | 61.8±30.1 (0/10) |
| RplA | 122.4±68.1 (6/10) | 92.1±32.5 (3/10) | 49.4±26.8 (0/10) | 17.7±21.3 (0/10) | 40.2±36.7 (0/10) | 38.7±26.8 (0/10) | 24.4±12.9 (0/10) | 38.1±24.4 (0/10) |
| RplY | 316.2±169.2 (9/10) | 114.9±76.5 (3/10) | 51.8±30.4 (0/10) | 35±28.6 (0/10) | 59.4±33.9 (1/10) | 35.3±29.9 (0/10) | 21.3±10 (0/10) | 40.6±27.1 (0/10) |
| RpsB | 658.8±348.5 (6/10) | 196.9±84.6 (0/10) | 138±73.9 (0/10) | 383.4±91.7 (2/10) | 233±152.6 (0/10) | 204.5±230.2 (1/10) | 240.1±59.6 (0/10) | 333.9±54.6 (0/10) |
| SdhB | 129.6±79.6 (8/10) | 101±41.8 (10/10) | 51.2±37 (5/10) | 31.8±28 (3/10) | 32.2±21.1 (1/10) | 40.4±33.3 (4/10) | 18±13.7 (1/10) | 22.2±8.6 (0/10) |
| SurA | 179.9±139.2 (7/10) | 110.1±43.9 (4/10) | 67.6±42 (1/10) | 25.1±13.7 (0/10) | 43.1±23.5 (0/10) | 52.2±50.9 (2/10) | 44.8±20 (0/10) | 49.4±18.6 (0/10) |
| YbgF | 185±128.1 (8/10) | 97.9±50.2 (3/10) | 49.8±33.9 (1/10) | 31.7±23.6 (0/10) | 31.6±22.8 (0/10) | 37.1±36.9 (1/10) | 23±13.9 (0/10) | 39.8±18.9 (0/10) |
| Rh054_02285 | 182.5±99.9 (8/10) | 111.3±44.1 (7/10) | 69.8±39.6 (4/10) | 57.3±29.8 (1/10) | 48.6±25.1 (1/10) | 38.7±27.1 (1/10) | 98.5±124.3 (3/10) | 46.8±11.7 (0/10) |
The major seroreactive surface-exposed proteins of R. heilongjiangensis fabricated on a microarray slide were probed with the sera from mice infected with R. heilongjiangensis (R. hei), R. sibirica (R. sib), R. rickettsii (R. ric), R. prowazekii (R. pro), R. typhi (R. typ), O. tsutsugamushi (O. tsu) or C. burnetii (C. bur), or normal mouse sera (Normal).