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. 2013 Jul 23;8(7):e70518. doi: 10.1371/journal.pone.0070518

Figure 1. The NTD is necessary for Mcm10 self-association.

Figure 1

(A) Schematic of Xenopus laevis Mcm10 constructs used to study self-association. Theoretical molecular masses are 95.4 kDa (Mcm10) and 70.4 kDa (Mcm10ΔN). (B) Sedimentation velocity data for full-length Mcm10 (black dotted line) and Mcm10ΔN (blue) as described in Materials and Methods. The molecular mass of the Mcm10ΔN major peak was calculated to be 68.8 kDa. Molecular masses could not be accurately determined from the full-length data. (C,D) SEC-MALS analysis of Mcm10 (C) and Mcm10ΔN (D). The UV trace is shown as a black dotted line and the light scattering trace is red. (C) Estimated molecular masses were calculated from the three shaded regions to be 90.4 kDa (I), 189.3 kDa (II), and 322.7 kDa (III). The peak at 18 min corresponds to the void volume. (D) The molecular mass of Mcm10ΔN was calculated to be 75.1±0.8 kDa.