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. 2013 Jul 23;8(7):e70177. doi: 10.1371/journal.pone.0070177

Figure 1. Specificity of the preproKiss2 antibody.

Figure 1

(A) Immunoblotting demonstrating that the antibody directed against the C-terminus of the preproKiss2 recognizes the peptide ELEVPT sequence and does not cross-react with the preproKiss1 derived sequences. (B–D) Cells co-transfected with pCDNA3-Kiss2 (red in B) and pCDNA3-GFP (green) expression vectors exhibit immunoreactivity to the preproKiss2 antibody. Controls transfected with the empty vector are negative. Bar = 15 µm. (E–F) Cells labelled by the preproKiss2 probes (green) using in situ hybridization also exhibit immunoreactivity for preproKiss2 (red). E: Bar = 25 µm; F: Bar = 10 µm.