Figure 2. Labeled cells in the cerebellar cortex by IUE of transposon plasmids.

(A) Low magnification view of a sagittal section of a Tol2-GFP electroporated cerebellum. (B) A basket cell extending its axon branches towards the Purkinje cell layer (lower left). (C) A stellate cell in the upper ML extending ramified processes. Black dots in the insets in B and C show the location of the respective cell bodies. (D) A granule cell with radiating dendrites (arrow) and an axon extending towards the ML (arrowheads). (E,F) Numerous parallel fibers occupy the ML. F is a high-magnification view of the boxed region in E. (G) A UBC in the IGL. (H) A Bergmann glia. (I) An astrocyte in the IGL. (J) An oligodendrocyte in the WM. (K) A large-diameter DN neuron (arrowhead). (L) A small-diameter DN neuron. (M1, N1, O1, P1) Maximum projection images for each series of confocal images of each cell. (M2–M4, N2–N4, O2–O4, P2–P4) Confocal plane images. (M1–M4) A Tol2-GFP labeled cell in the upper part of the ML shows the morphology of stellate cells. Double-labeling immunohistochemistry for parvalbumin (magenta) and GFP (green) confirmed the stellate cell identity. (N1–N4) A Tol2-GFP labeled cell in the lower part of the ML shows the morphology of basket cells. Double-labeling immunohistochemistry for parvalbumin (magenta) and GFP (green) confirmed the basket cell identity. (O1–O4) A Tol2-GFP labeled cell in the IGL shows the morphology of astrocytes. Double-labeling immunohistochemistry for GFAP (magenta) and GFP (green) confirmed the astrocyte identity. (P1–P4) A Tol2-GFP labeled cell in the white matter shows the morphology of oligodendrocytes. Double-labeling immunohistochemistry for MBP (magenta) and GFP (green) confirmed the oligodendrocyte identity. Scale bars: 10 µm ML, molecular layer; PCL, Purkinje cell layer; IGL, internal granular layer. Scale bars: A, 1 mm; B, C, E, F, K, L, M1, N1, O1, and P1, 10 µm; in G, 10 µm for D and G; in J, 10 µm for H, I, and J.