Figure 5. Destabilizing effect of mutation E374K.
A, wild-type HA; B, E374K mutant. Protein is shown as green ribbons. Amino acids surrounding position 374 are illustrated as sticks. In the wild-type HA, E374 interacts with Y433 and N366 and with the fusion peptide through water-mediated hydrogen bonds. In E374K, the lysine residue interacts only with the oxygen from the amide group of N366.