Figure 2. Effect of reaction temperature on phospholipid-mediated BH formation.
Dipalmitoyl-PC (A), dimyristoyl-PE (B), dimyristoyl-PC (C), and dioleoyl-PC (D) were used to induce BH formation at various temperatures, and the reaction was dependent on the phase transition temperature of phospholipids. T m values are 42°C for dipalmitoyl-PC (A), 48°C for dimyristoyl-PE (B), 23°C for dimyristoyl-PC (C), and −22°C for dioleoyl-PC (D). It is noted that BH was not formed at 50°C in the absence of phospholipids. Values represent the mean ± SD (n = 3).
