Table 3.
Effect of MMC + LA on the frequency of micronucleus and cytokinesis block proliferation index in cultured human lymphocytes
| Test substance | Treatment | BN cells scored | Distrubition of BN cells according to the no of MN | MN (%) ± SE | Cytokinesis-block proliferation index (CBPI) ± SE | ||||
|---|---|---|---|---|---|---|---|---|---|
| Time (hour) | Dose (μg/ml) | ||||||||
| MMC | LA | ||||||||
| (1) | (2) | (3) | |||||||
| Negative control | 48 | – | – | 2,000 | 4 | – | – | 0.20 ± 0.009 | 1.95 ± 0.043 |
| Solvent control | 48 | – | – | 2,000 | 9 | 1 | – | 0.55 ± 0.011 | 1.93 ± 0.042 |
| Positive control (MMC) | 48 | 0.2 | – | 2,000 | 153 | 9 | – | 8.55 ± 0.18 | 1.86 ± 0.40 |
| MMC + LA (1 h pre-treatment) | 49 | 0.2 | 0.5 | 2,000 | 98 | 2 | – | 5.10 ± 0.10 ** | 1.87 ± 0.040 |
| 0.2 | 1 | 2,000 | 100 | 8 | – | 5.80 ± 0.12 ** | 1.68 ± 0.034** | ||
| 0.2 | 2 | 2,000 | 100 | 15 | – | 6.75 ± 0.14 ** | 1.52 ± 0.028** | ||
| MMC + LA (simultaneous treatment) | 48 | 0.2 | 0.5 | 2,000 | 130 | 8 | – | 7.30 ± 0.15 ** | 1.90 ± 0.041 |
| 0.2 | 0.5 | 2,000 | 123 | 8 | – | 6.95 ± 0.14 ** | 1.74 ± 0.036* | ||
| 0.2 | 0.5 | 2,000 | 118 | 12 | 4 | 7.70 ± 0.16 | 1.58 ± 0.030** | ||
| MMC + LA (1 h post-treatment) | 47 | 0.2 | 1 | 2,000 | 122 | 8 | – | 6.90 ± 0.14 ** | 1.78 ± 0.037 |
| 0.2 | 2 | 2,000 | 126 | 14 | – | 7.70 ± 0.16 ** | 1.68 ± 0.034** | ||
| 0.2 | 1 | 2,000 | 133 | 9 | – | 7.55 ± 0.16 ** | 1.58 ± 0.030** | ||
* Significantly different at p < 0.01 when compared with positive control (z test)
** Significantly different at p < 0.001 when compared with positive control (z test)
MMC Mitomycin-C, LA Lipoic acid, BN Binucleat, MN micronucleus, SE standard error