Fig. 3.

Soft agar colony formation analysis and expression of cell cycle and apoptosis related genes in HeLa or U14 cells after treatment with reversine and aspirin for 48 h a HeLa and U14 cells colony formation decreased significantly in the combination group. b Quantitative analysis of soft agar clone formation after different treatments, P < 0.05. c qRT PCR analysis of the expression level of cell cycle and apoptosis related genes, Cyclin D1 and Bcl-2 decreased while p21, Caspase-3 and Bax expression increased in the combination group compared with the drugs used separately, P < 0.05. d Expression of genes related to cell cycle and apoptosis by Western blotting. e Scanning densitometric analysis of Western blotting