Fig. 2.

Inhibition of LLC cell migration by combination of VPA and HCCA. The effects of combination of VPA and HCCA on the migration of LLC cells were examined by the migration assay as described in the section of “Materials and methods”. The enhanced inhibition of LLC cell migration by combination of VPA and HCCA is shown in (a) and (b). a The photos (200X) show the propidium iodide-stained LLC cells that migrated through fibronectin-coated transwell chamber. b The cells were treated for 6 h with the indicated concentrations of VPA/V and HCCA/C (V0.125/0.125 mM, V0.25/0.25 mM, V0.5/0.5 mM, C8/0.008 mM, C16/0.016 mM). The control (0) received DMSO vehicle. The data are presented as the mean ± SD (Bar) (n = 6). The figure is the representative of 3 similar experiments performed. *P < 0.05. Values with different letters (a–f) differ significantly (P < 0.05); c/s, d/s, e/s and f/s represent the significant enhanced effects (P < 0.05) compared with VPA or HCCA alone