Fig. 1.

The scheme shows the gene construct pGal-GFPBsd encompassing a strong, constitutive EF-1α promoter (1,179 bp), cDNA of the gene coding for human α-galactosidase (1,290 bp) and the poly(A) sequence of the cattle GH gene (225 bp). Moreover, the vector contains the sequence of Bsd gene coding for blasticidin antibiotic resistance joined with the GFP sequence under the control of the CMV promoter. The scheme also shows the locations of the primers used for the screening of the transgene (F1 and R1, F2 and R2) and the Southern probe binding site (between F1 and R1 primers)