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. 2013 Jun 19;54(3):293–303. doi: 10.1007/s13353-013-0156-y

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© The Author(s) 2013

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Fig. 2 — Screening of the pGal-GFPBsd gene construct. Polymerase chain reaction (PCR) was used to amplify DNA fragments of 464 bp and 861 bp. The separation of DNA fragments was conducted in 1.5 % agarose gel. a Analysis of the integration with genomic DNA in pigs (F0). Line 1, size marker (λ DNA/HindIII, EcoRI); lane 2, DNA isolated from TG252; lane 3, DNA isolated from 253; lane 4, negative control (without DNA); lane 5, negative control (wild-type pig DNA); lane 6, positive control (pGal-GFPBsd gene construct). b Analysis of integration with genomic DNA in pigs (F1). Lanes 1–5 and 7–13, DNA isolated from potentially transgenic pigs; line 6, size marker (λ DNA/HindIII, EcoRI); line 14, negative control (wild-type pig DNA); line 15, negative control (without DNA); line 16, positive control (pGal-GFPBsd gene construct)