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. 2013 Jun 19;54(3):293–303. doi: 10.1007/s13353-013-0156-y

Fig. 5.

Fig. 5

Transgene expression analysis by flow cytometry. Fibroblasts derived from the following animals were analysed: wild-type, pig TG252 (F0), transgenic pigs F1 (TG1183, TG1036, TG1040), TG1154 expressing 1,2-fucosyltransferase labelled by BS-IB4 lectin (detects Gal epitope), conjugated with AlexaFluor 647 fluorochrome. a The x-axis shows the fluorescence intensity, the y-axis shows the number of cells which were not labelled (black lines), labelled wild-type cells (red line), transgenic animal TG252 (blue line) and transgenic animal TG1154 (green line). The analysis showed decreased levels of Gal antigen in transgenic animals compared to wild-type animals. The fluorescence fell by 58.9 % for the pig with α-galactosidase (TG252) expression and by 62.8 % for the pig with α1,2-fucosyltransferase (TG1154) expression compared to control. b The x-axis shows the fluorescence intensity, the y-axis shows the number of cells which were not labelled (black lines), labelled wild-type cells (red line), transgenic animal TG252 (F0) (light green line) and transgenic animal TG1183 (F1) (dark blue line), transgenic animal TG1036 (F1) (light blue line) and transgenic animal TG1040 (F1) (dark green line). The analysis showed decreased levels of Gal antigen in transgenic animals in F0 and F1 compared to wild-type animals. The reduction of Gal epitope expression was, respectively, 58.21 %, 59.67 %, 56.67 % and 65.09 %. The level of Gal epitope expression observed in the F1 animals was similar to the F0 animal (TG252)