Table 1.
Adjustment of dissolved oxygen (dO2) and corresponding values of OD565 and Cmag during submerged culture of MSR-1 cells.
| Time (h) | Stirring rate (rpm) | Airflow rate | dO2 | OD565 | Cmag | |
|---|---|---|---|---|---|---|
| (L/min) | (%) | (ppb) | ||||
| 0 | 120 | 0.94 | 102.70 | 2451 | 0.1 | 0.74 |
| 8 | 120 | 0.95 | 0.00 | 12 | 0.3 | 0.24 |
| 10 | 140 | 1.45 | 0.00 | 4 | 0.4 | 0.80 |
| 12 | 160 | 1.95 | 0.00 | 4 | 0.7 | 1.39 |
| 14 | 180 | 1.97 | 0.00 | 3 | 1.1 | 1.38 |
| 16 | 180 | 1.95 | 0.00 | 2 | 1.5 | 1.65 |
| 18 | 200 | 1.95 | 0.00 | 3 | 1.7 | 1.52 |
| 20 | 230 | 2.95 | 0.00 | 6 | 2.1 | 1.78 |
| 22 | 250 | 3.98 | 0.00 | 4 | 3.2 | 1.65 |
| 24 | 270 | 3.96 | 0.00 | 17 | 4.8 | 1.71 |
| 26 | 280 | 3.99 | 0.00 | 35 | 6.3 | 1.38 |
| 28 | 290 | 3.98 | 0.00 | 9 | 8.3 | 1.50 |
| 30 | 300 | 3.97 | 0.00 | 215 | 10.8 | 1.54 |
| 32 | 300 | 1.97 | 0.00 | 186 | 13.7 | 1.68 |
| 34 | 300 | 1.95 | 0.00 | 204 | 14.6 | 1.41 |
| 36 | 300 | 1.95 | 12.40 | 661 | 16.8 | 1.43 |
| 38 | 300 | 1.95 | 21.60 | 962 | 18.1 | 1.20 |
| 40 | 300 | 1.95 | 29.80 | 1247 | 19.4 | 1.12 |
dO2 was measured using two probes with differing accuracies (see Materials and Methods). To maintain dO2 at the level suitable for magnetosome synthesis (0.0%), the stirring rate was changed at 10, 12, 14, 18, 20, 22, 24, 26, 28, and 30 h, and the airflow rate was changed at 10, 12, 20, 22, and 32 h.