Table 1. Growth conditions for B. cenocepacia transcriptomic reference set.
| Experimenta | Test condition | Control condition (control number)b |
|---|---|---|
| Stationary phase in minimal medium | BSM | Log phase in BSM (4) |
| Stationary phase in nutrient-rich medium | LB | Log phase in LB (1) |
| Low oxygen concentration | LB, atmosphere with 6% oxygen | Fully aerated LB (2) |
| Oxidative stress (inorganic peroxide) | LB, 0.15% H2O2, 15 min | LB without added peroxide (3) |
| Oxidative stress (organic peroxide) | LB, 0.001% tertiary butyl hydroperoxide, 15 min | LB without added peroxide (3) |
| Heat stress | LB, 42.5 °C, 1 h | LB at 37 °C (3) |
| Physiological temperature | LB, 20 °C | LB at 37 °C (2) |
| Low iron concentration | BSM, Fe content 0.064 p.p.m. (1.2 μℳ) | BSM, Fe content 2.45 p.p.m.=44 μℳ (4) |
| Low pH | BSM, pH 5.5 | BSM, pH 7.0 (4) |
Abbreviations: BSM, basal salts medium; LB, Luria Bertani broth
a
Cultures were incubated fully aerated shaking at 150 r.p.m. and at 37 °C if not stated otherwise.
b
Controls we run for each condition as indicated by the control number; this number also corresponds to the control conditions shown in Figure 1.