Table 1. Separate statistical analysis (CCA, CCA per groups and PERMANOVA) to estimate the contribution (from R 2 values) of five environmental factors in the ARISA fingerprinting variance.
Variable |
CCA |
CCA (separate temperature groups) |
PERMANOVA |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
COLD |
TEMPERATE |
WARM |
|||||||||
R2 | Sign | R2 | Sign | R2 | Sign | R2 | Sign | Grouped factors | R2 | Sign | |
Temperature (°C) | 0.51 | *** | 0.01 | 0.12 | ** | 0.21 | *** | 2–9.5, 9.5–17, 17–30 | 0.36 | *** | |
Z (m) | 0.18 | *** | 0.02 | 0.41 | *** | 0.13 | *** | 0–25, 25–75, 75–180, 180–250 | 0.08 | *** | |
Salinity | 0.11 | *** | 0.06 | ** | 0.10 | * | 0.25 | *** | 32, 33, 34, 35, 36, 37, 38, 39 | 0.07 | *** |
Zmax (m) | 0.06 | *** | 0.01 | 0.06 | 0.08 | ** | 0–200, 200–1000, 1000–6000 | 0.02 | * | ||
Chlorophyll (μg l−1) | 0.05 | * | 0.01 | 0.04 | 0.15 | *** | 0–0.5, 0.5–1.5, 1.5–4 | 0.02 | * | ||
Total | 0.91 | 0.06 | 0.63 | 0.82 | 0.55 |
Abbreviations: ARISA, automated ribosomal intergenic spacer analysis; CCA, Constrained Correspondence Analysis; PERMANOVA, permutational multivariate analysis of variance; Z, sampling depth; Zmax, bottom depth.
Significance codes: ***<0.001; **0.001–0.01; *0.01–0.05.
Bold numbers represent the highest R2 of the particular analysis.