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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Parasitology. 2012 Oct 12;140(3):303–308. doi: 10.1017/S003118201200159X

Figure 2.

Figure 2

Example of 24Sα-rDNA PCR product size polymorphism A) First round PCR, primers D75 and D76 Lane (1) 100 bp ladder; lane (2) reference stock PAH 265 (TcV); lane (3) reference stock CL-Brener (TcVI); lane (4) reference stock Tu 18 (TcII); lane (5) sample of dog with identified DTU (TcV); lane (6) sample of cat with identified DTU (TcV); lane (7) sample of dog with identified DTU (TcVI); lane (8) sample of cat with identified DTU (TcVI); lane (9) 100 bp ladder. B) Heminested PCR, primers D71 and D76 Lane (1) sample of dog with identified DTU (TcIII); lane (2) reference stock M5631 (TcIII); lane (3) reference stock Can III (TcIV); lane (4) 100 bp ladder; lane (5) sample of dog with TcVI identified; lane (6) reference stock Tu 18 (TcII); lane (7) sample of cat with identified TcVI; lane (8) reference stock CL-Brener (TcVI); lane (9) reference stock PAH 265 (TcV); lane (10) sample of dog with TcV identified; lane (11) sample of cat with TcV identified.