Table 1.
Minor and major TRAF2-binding motifs and TRAF6-binding motifs in NLR proteins
| NLR | NCBI reference sequence | TRAF2 (minor) | TRAF2 (major) | TRAF6 |
|---|---|---|---|---|
| CIITA | NP_000237.2 | 57 (AGEE); 674 (TLQE); 910 (AAEE); 1115 (SVQE) | 29 (PLEGGY); 337 (PVEQFY) | |
| NAIP | NP_004527.2 | 232 (SSEE); 276 (AYEE); 448 (PVQE); 746 (AFQE); 1068 (AEQE); 1219 (PDEE) | ||
| Nod1 | AAD43922.1 | 479 (TQEE) | ||
| Nod2 | NP_071445.1 | 106 (AAQE); 1024 (SLEE) | ||
| NLRC3 | NP_849172.2 | 457 (SLQE); 582 (SVEE) | ||
| NLRC4 | AAH31555.1 | 445 (SFQE); 541 (TEQE) | ||
| NLRC5 | NP_115582.3 | 516 (SLQE); 1210 (SNEE); 1429 (PRQE); 1607 (SLEE) | 273 (PSELLF) | |
| NLRP1 | AAH51787.1 | 994 (PTEGLD) | 169 (PSQE); 1047 (SHEE) | 1247 (PEEVTF) |
| NLRP2 | AAH39269.1 | 612 (SQEE) | ||
| NLRP3 | NP_004886.3 | 112 (SIEE); 179 (SQQE); 524 (TFQE); 1020 (TLQE) | 689 (PKEEEE) | |
| NLRP4 | NP_604393.2 | 51 (SREE); 232 (SFEE); 971 (TAEE) | 284 (PKELRD) | |
| NLRP5 | NP_703148.4 | 244 (AEEE); 706 (SFQE) | ||
| NLRP6 | NP_612202.1 | 25 (ALEE); 158 (APEE); 165 (PAEE); 501 (SFQE); 606 (PEEE); 857 (SLQE) | 165 (PAEEPE); 606 (PEEEEE); 618 (PLELLY) | |
| NLRP7 | AAI09126.1 | 505 (SGEE); 578 (SQEE) | ||
| NLRP8 | NP_789781.2 | 49 (SHEE); 497 (AGEE); 509 (TFQE) | ||
| NLRP9 | NP_789790.2 | 88 (AQEE); 499 (STEE); 964 (SVEE) | ||
| NLRP10 | NP_789791.1 | 143 (PEQE); 257 (PFEE); 316 (SEEE); 612 (PKEE) | ||
| NLRP11 | NP_659444.2 | 48 (TKEE) | ||
| NLRP12 | NP_653288.1 | 202 (PDEE); 516 (SFQE) | ||
| NLRP13 | NP_789780.2 | 38 (PQQLMD) | 117 (PTQE); 140 (PNQE); 163 (PNQE); 295 (PIEE); 538 (SFQE); 645 (SQEE) | |
| NLRP14 | NP_789792.1 | 91 (AKEE); 243 (PIEE); 267 (AFEE); 334 (AREE) | 67 (PGEKAW) | |
| NLRX1 | AAI10891.1 | 672 (PSELLD) | 94 (PREE); 459 (TEEE); 496 (AMQE) | 262 (PEEPQE); 611 (PDEPPE) |
Sequences of TRAF-binding motifs in various NLR proteins; numbers before sequences (in one-letter code) correspond to first amino acid of motif. P-X-(Q/E)-X-X-D is the minor TRAF2-binding motif (and is the TRAF6-binding motif if third residue is Glu (E)); (P/S/A/T)-X-(Q/E)-E is the major TRAF2-binding motif; and P-X-E-X-X-aromatic (F/W/Y)-acidic (D/E)) is the TRAF6-binding motif (where ‘X’ indicates any amino acid and ‘/’ indicates ‘or’). The minor TRAF2-binding motifs for NLRP1 and NLRX1 may also be TRAF6-binding motifs.