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. 2013 May 29;11(6):1853–1865. doi: 10.3390/md11061853

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Effect of isolates (10 μM) from S. gracillimum on the lipopolysaccharide (LPS)-induced pro-inflammatory iNOS and on COX-2 protein expression of RAW264.7 macrophage cells by immunoblot analysis. (A) Immunoblots iNOS and β-actin; (B) Immunoblots COX-2 and β-actin. The values are means ± SEM (n = 6). The relative intensity of the LPS alone stimulated group was taken as 100%. * Significantly different from LPS alone stimulated group (* p < 0.05). ^a stimulated with LPS; ^b stimulated with LPS in the presence of 1, 2, and 4–6 (10 μM).