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. 2013 Jun 3;11(6):1899–1908. doi: 10.3390/md11061899

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Lycogen reduces melanin content through the ERK pathway. B16F10 cells were treated with Lycogen™ at the concentration of 12.5 μM for 48h and PD98059 (50 μM) for 24h. (a) The protein and (b) melanin expression weremeasured. The expression of β-actin served as the quantitative control. Inserted values indicated relative protein expression in comparison with β-actin. (c) The melanin was detected in B16F10 cells transiently transfected withERK dominant negative or with control vector after treatment with Lycogen™.