Figure 3.

Sperm cells, at T₀ or incubated for 180 min in capacitating conditions with increasing concentrations of Asta, or 1 μM Diam, were analyzed for Tyr-P pattern, acrosome-reacted cells (ARC) and viability (non-viable cells (NVC)) by immunofluorescence cytochemistry (see Methods). Number of cells expressed as % of total number of cells showing Tyr-P in any part of cell body or in head, were detected and reported as Tyr-P cells and Tyr-P head, respectively. Percentages of cells undergoing acrosome reaction (AR) or NVC were also reported. Values are expressed as means ± SD. † p < 0.01; ‡ p < 0.0001 comparison between various concentrations of Asta or Diam vs. CC as reference; Student’s t-test for paired data. * p < 0.01; ** p < 0.0001 comparison CC vs. T₀; Student’s t-test for paired data.