Figure 1. Transfer is a highly efficient OM-specific process.

(A) Percentage of mCherry⁺ recipient cells as a function of time. For each strain and time point, at least 3000 cells were analyzed in triplicate. Error bars = SD. (B) Fluorescence intensity of mCherry⁺ recipient cells as a function of time. For each time point, the fluorescence numbers are expressed as a percentage of the mean fluorescence intensity of the donor cells population. For each time point, fluorescence intensities were measured for ∼3000 cells per strain. (C) Distribution of fluorescence intensities measured in the positive recipient cells after 12 hr (green bars) and 36 hr (orange bars) of co-incubation. Note the logarithmic scale log(Fluorescence Intensity). The black arrow highlights the appearance of a highly-stained cell sub-population of mCherry⁺ cells at 36 hr. For each time point, fluorescence intensities were measured for ∼3000 cells per strain.

DOI: http://dx.doi.org/10.7554/eLife.00868.003

Figure 1—figure supplement 1. Subcellular localization of indicated fluorescent probes before and after a plasmolysis treatment.

(A and B) Sub-cellular localization of the OM_mcherry, OM_sfGFP, IM_mcherry, PERI_mcherry fusions before (−) and after (+) plasmolysis treatment (0.5 M NaCl). For each fusion, cells were immobilized in a hybrid flow chamber and imaged before and after injection of the plasmolysis solution. Note that fluorescent cytoplasmic aggregates are observed for the IM_mcherry fusion after the plasmolysis treatment (white arrow). Scale bar = 1 µm.

DOI: http://dx.doi.org/10.7554/eLife.00868.004